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Isntree Tw- Real Bifida Ampoule

Tw- Real Bifida Ampoule (Discontinued)

BARRIER REPAIR + ANTI-WRINKLE BRIGHTENING 88% Bifida ferment lysate, 3 peptides to help firm and brighten skin
Uploaded by: evi on

Skim through

Ingredient name what-it-does irr., com. ID-Rating
Bifida Ferment Lysate goodie
Dipropylene Glycol solvent, perfuming, viscosity controlling
Niacinamide cell-communicating ingredient, skin brightening, anti-acne, moisturizer/​humectant superstar
Glycerin skin-identical ingredient, moisturizer/​humectant 0, 0 superstar
Lactobacillus/Pumpkin Ferment Extract exfoliant goodie
Lactobacillus/Soybean Ferment Extract
Rice Ferment Filtrate (Sake)
Sodium Hyaluronate skin-identical ingredient, moisturizer/​humectant 0, 0 goodie
Hydrolyzed Collagen emollient, moisturizer/​humectant goodie
Salix Alba (Willow) Bark Extract soothing goodie
Origanum Vulgare Leaf Extract
Cinnamomum Cassia Bark Extract
Portulaca Oleracea Extract soothing, antioxidant goodie
Chamaecyparis Obtusa Leaf Extract
Beta-Glucan soothing, moisturizer/​humectant goodie
Palmitoyl Oligopeptide cell-communicating ingredient goodie
Copper Tripeptide-1 cell-communicating ingredient, soothing, antioxidant goodie
Acetyl Hexapeptide-8 cell-communicating ingredient, moisturizer/​humectant goodie
Allantoin soothing 0, 0 goodie
Hydroxyethylcellulose viscosity controlling
Panthenol soothing, moisturizer/​humectant 0, 0 goodie
Carbomer viscosity controlling 0, 1
Arginine skin-identical ingredient goodie
Butylene Glycol moisturizer/​humectant, solvent, viscosity controlling 0, 1
Scutellaria Baicalensis Root Extract soothing, antioxidant, antimicrobial/​antibacterial, moisturizer/​humectant goodie
Illicium Verum (Anise) Fruit Extract perfuming
Adenosine cell-communicating ingredient goodie
1,2-Hexanediol solvent
Disodium EDTA chelating

Isntree Tw- Real Bifida Ampoule
Ingredients explained

Bifida Ferment Lysate is a probiotic ingredient that’s used in one of the most iconic serums in the world, the Estee Lauder Advanced Night Repair. Based on manufacturer info it potently protects against UV-induced damage in the skin and helps with DNA repair. An Estee Lauder patent from 2009 also talks about DNA repair activity.

As far as published research goes we could find one promising one. It showed that applying 10% bacterial extract to sensitive, reactive skin helped with better barrier function and decreased skin sensitivity

A clear, colorless liquid that works as a solvent and viscosity decreasing ingredient. It also has great skin-moisturizing abilities. 

Niacinamide - superstar
Also-called: vitamin B3, nicotinamide | What-it-does: cell-communicating ingredient, skin brightening, anti-acne, moisturizer/humectant
  • A multi-functional skincare superstar with several proven benefits for the skin
  • Great anti-aging, wrinkle smoothing ingredient used at 4-5% concentration
  • Fades brown spots alone or in combination with amino sugar, acetyl glucosamine
  • Increases ceramide synthesis that results in a stronger, healthier skin barrier and better skin hydration
  • Can help to improve several skin conditions including acne, rosacea, and atopic dermatitis
Read all the geeky details about Niacinamide here >>

Glycerin - superstar
Also-called: Glycerol | What-it-does: skin-identical ingredient, moisturizer/humectant | Irritancy: 0 | Comedogenicity: 0
  • A natural moisturizer that’s also in our skin
  • A super common, safe, effective and cheap molecule used for more than 50 years
  • Not only a simple moisturizer but knows much more: keeps the skin lipids between our skin cells in a healthy (liquid crystal) state, protects against irritation, helps to restore barrier
  • Effective from as low as 3% with even more benefits at higher concentrations up to 20-40% (around 10% is a good usability-effectiveness sweet spot)
  • High-glycerin moisturizers are awesome for treating severely dry skin
Read all the geeky details about Glycerin here >>

Also-called: Pumpkin Enyzme | What-it-does: exfoliant

When you hear pumpkin you probably think of Halloween or autumn, but it turns out that many forms of pumpkin are also great skincare ingredients.

This particular form is obtained by fermenting the pumpkin fruit with lactobacillus lactis. During the fermentation, many of the complex biomolecules are broken down into isolated phytochemicals and selective filtration techniques are used to isolate the proteolytic parts.  The result is pumpkin enzyme that is a gentle exfoliator that can be an alternative to AHAs

We don't have description for this ingredient yet.

We don't have description for this ingredient yet.

What-it-does: skin-identical ingredient, moisturizer/humectant | Irritancy: 0 | Comedogenicity: 0

It’s the - sodium form - cousin of the famous NMFhyaluronic acid (HA). If HA does not tell you anything we have a super detailed, geeky explanation about it here.  The TL; DR version of HA is that it's a huge polymer (big molecule from repeated subunits) found in the skin that acts as a sponge helping the skin to hold onto water, being plump and elastic. HA is famous for its crazy water holding capacity as it can bind up to 1000 times its own weight in water.

As far as skincare goes, sodium hyaluronate and hyaluronic acid are pretty much the same and the two names are used interchangeably. As cosmetic chemist kindofstephen writes on reddit  "sodium hyaluronate disassociates into hyaluronic acid molecule and a sodium atom in solution". 

The chemically chopped up version of the big protein molecule, collagen. It is often derived from fish or bovine sources and works as a nice moisturizer and humectant that helps the skin to hold onto water.  

To understand a bit more what Hydrolyzed Collagen is, you have to know that proteins are large chains of amino acids connected with so-called peptide bonds. These bonds can be broken up when a water molecule is added and the resulting thing is a mix of shorter length amino acids, also called peptides. So Hydrolyzed Collagen is not really collagen, it is rather an undefined and varying mix of largish peptides. Based on a manufacturer's data, the whole, soluble collagen has an average molecular weight of 300 000 Da, while this chopped up mixture has an average MW of 12 000 Da (still pretty big). 

Also-called: White Willow Bark Extract | What-it-does: soothing, astringent

The extract coming from the bark of the White Willow, a big (25 m/80 ft.) tree that likes to live on riverbanks. It's famous for containing anti-inflammatory natural salicylates (this powder, for example, is standardized to contain 53-65%), a close chemical relative to famous exfoliant salicylic acid.

Thanks to its salicin content, willow bark is often touted as a natural alternative to salicylic acid, though it's quite questionable how effective it is as a chemical exfoliant in the tiny amounts used in cosmetics. Apart from soothing salicin, it also contains flavonoids and phenolic acids that give willow bark tonic, astringent, and antiseptic properties.

We don't have description for this ingredient yet.

We don't have description for this ingredient yet.

Also-called: Purslane Extract | What-it-does: soothing, antioxidant

Portulaca Oleracea is a nice succulent with bright yellow flowers and edible nutritious vegetables. It's a famous plant in Korean traditional medicine to treat infection and irritated skin.

Modern research confirms that it's loaded with skin-goodies: it's the richest green plant source of omega-3 fatty acids (α-linolenic acid), contains NMFs (polysaccharides and amino acids),  vitamins (β-carotene), minerals, and antioxidants (yellow betaxanthins and reddish betacyanins). Thanks to all its beneficial components, Purslane Extract has several magic properties: it's a great anti-inflammatory and antioxidant agent and also has wound healing abilities

We don't have description for this ingredient yet.

Beta-Glucan - goodie

Beta-Glucan is a nice big molecule composed of many smaller sugar molecules (called polysaccharide). It’s in the cell walls of yeast, some mushrooms, seaweeds, and cereals.

It’s a real goodie no matter if you eat it or put it on your face. Eating it is anti-diabetic, anti-cancer, and even lowers blood cholesterol. 

Also-called: Biopeptide El, pal-VGVAPG, Palmitoyl Oligopeptide (old name);Palmitoyl Hexapeptide-12 | What-it-does: cell-communicating ingredient

A six amino acid peptide that is claimed to improve firmness and skin tone. Its amino acid sequence is Val-Gly-Val-Ala-Pro-Gly that is also called the "spring fragment" and is repeated six times in the important skin protein, elastin molecule.

The manufacturer made a double-blind, one-month long clinical study on 10 women and found that twice a day application of 4%  Biopeptide El improved skin firmness by 33% and skin tone by 20%. 

Also-called: GHK-Cu, Copper Peptide | What-it-does: cell-communicating ingredient, soothing, antioxidant
  • GHK-Cu is a copper-peptide complex found naturally in yucky bodily fluids (plasma, saliva, and urine)
  • It has unique wound healing properties by stimulating the breakdown of unhealthy, too large collagen in scar tissue and stimulating nice and healthy collagen production afterwards
  • It stimulates the production of several important skin-identical ingredients (e.g. collagen, elastin, and glycosaminoglycans)
  • It has significant anti-inflammatory and antioxidant effect
  • There are a few promising studies showing GHK-Cu can improve skin elasticity, clarity, firmness and reduce lines and wrinkles
  • It can improve hair growth by enlarging hair follicles (and bigger follicles produce longer, thicker hair)
  • There are some urban legends about possible overdosing or facial hair problems (read more in geeky details)
Read all the geeky details about Copper Tripeptide-1 here >>

Also-called: Argireline, Acetyl Hexapeptide-3 | What-it-does: cell-communicating ingredient, moisturizer/humectant

If you see a cosmetic product that claims that it has "Botox-like effect" then two things are almost certain: one, the product overpromises and two, it contains Argireline. 

So this one is the famous peptide that's marketed by its manufacturer as the "Botox in a jar". The basis for this claim is that it targets the same wrinkle forming mechanism (wrinkles caused by facial muscle movement) as Botox, but the way it works is very different. In addition, the extent to which it can prevent muscles from contracting (and to smooth wrinkles) is very different (otherwise why would anyone use still Botox?). 

Allantoin - goodie
What-it-does: soothing | Irritancy: 0 | Comedogenicity: 0

Super common soothing ingredient. It can be found naturally in the roots & leaves of the comfrey plant, but more often than not what's in the cosmetic products is produced synthetically. 

It's not only soothing but it' also skin-softening and protecting and can promote wound healing.

Also-called: HEC | What-it-does: viscosity controlling, emulsion stabilising

A nice little helper ingredient that can thicken up cosmetic products and create beautiful gel formulas. It's derived from cellulose, the major component of the cell wall of green plants. It is compatible with most co-ingredients and gives a very good slip to the formulas. 

Panthenol - goodie
Also-called: Pro-Vitamin B5 | What-it-does: soothing, moisturizer/humectant | Irritancy: 0 | Comedogenicity: 0

An easy-to-formulate, commonly used, nice to have ingredient that’s also called pro-vitamin B5. As you might guess from the “pro” part, it’s a precursor to vitamin B5 (whose fancy name is pantothenic acid). 

Its main job in skincare products is to moisturise the skin. It’s a humectant meaning that it can help the skin to attract water and then hold onto it. There is also research showing that panthenol can help our skin to produce more lovely lipids that are important for a strong and healthy skin barrier. 

What-it-does: viscosity controlling, emulsion stabilising | Irritancy: 0 | Comedogenicity: 1

A big molecule created from repeated subunits (a polymer of acrylic acid) that magically converts a liquid into a nice gel formula.  It usually has to be neutralized with a base (such as sodium hydroxide) for the thickening to occur and it creates viscous, clear gels that also feel nice and non-tacky on the skin. No wonder, it is a very popular and common ingredient. Typically used at 1% or less in most formulations.

Arginine - goodie

A semi-essential (infants cannot synthesize it, but adults can) amino acid that is one of the primary building blocks of hair keratin and skin collagen. It's a natural moisturizing factor, a skin hydrator and might also help to speed up wound healing

Arginine usually has a positive charge (cationic) that makes it substantive to skin and hair (those are more negatively charged surfaces) and an excellent film former.  Thanks to the positive charge, it also creates a complex with AHAs (AHAs like to lose a hydrogen ion and be negatively charged, so the positive and the negative ions attract each other) that causes a "time-release AHA effect" and reduces the irritation associated with AHAs

What-it-does: moisturizer/humectant, solvent, viscosity controlling | Irritancy: 0 | Comedogenicity: 1

Butylene glycol, or let’s just call it BG, is a multi-tasking colorless, syrupy liquid. It’s a great pick for creating a nice feeling product.  

BG’s main job is usually to be a solvent for the other ingredients. Other tasks include helping the product to absorb faster and deeper into the skin (penetration enhancer), making the product spread nicely over the skin (slip agent), and attracting water (humectant) into the skin.

Also-called: Skullcap Root Extract | What-it-does: soothing, antioxidant, antimicrobial/antibacterial, astringent, moisturizer/humectant

A traditional Chinese herbal medicine loaded with potent antioxidant and anti-inflammatory flavonoids such as baicalin, baicalein, and wogonin.

If that would not be enough, Skullcap Root is also claimed to have antimicrobial and antifungal properties (also against P.acnes and Malassezia furfur) as well as some skin-brightening activity. A multi-functional skin-goodie.

What-it-does: perfuming

We don't have description for this ingredient yet.

Adenosine - goodie

Adenosine is an important little compound in our body that has a vital cell-signalling role. Research on smearing it on our face is also promising and shows so far a couple of things:

  • It can help with wound healing
  • It’s a good anti-inflammatory agent
  • It might even help with skin’s own collagen production and improve skin firmness and elasticity
  • It helps with barrier repair and protection
  • It might be even useful for the hair helping with hair thickness and hair growth
What-it-does: solvent

A really multi-functional helper ingredient that can do several things in a skincare product: it can bring a soft and pleasant feel to the formula, it can act as a humectant and emollient, it can be a solvent for some other ingredients (for example it can help to stabilize perfumes in watery products) and it can also help to disperse pigments more evenly in makeup products. And that is still not all: it can also boost the antimicrobial activity of preservatives

What-it-does: chelating

Super common little helper ingredient that helps products to remain nice and stable for a longer time. It does so by neutralizing the metal ions in the formula (that usually get into there from water) that would otherwise cause some not so nice changes.

It is typically used in tiny amounts, around 0.1% or less.

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